Bacterial Cadmium-Immobilization Activity Measured by Isothermal Microcalorimetry in Cacao-Growing Soils From Colombia

In cacao farms, the presence of cadmium (Cd) is a major issue for commercialization, particularly countries such as Colombia. Cadmium-tolerant bacteria (CdtB) are an important functional group microorganisms with potential bioremediation strategies. Cd immobilization activity by CdtB can be accurately measured isothermal microcalorimetry (IMC). this study, metabolic capacity entire community in farm soils from three cacao-producing districts Colombia, and without addition was using IMC. The differences between observed peaks related to were analysed determine whether activation populations occurred when content increased. thermograms Santander soil samples have compared maximal heat-flow Antioquia Arauca. IMC showed ratios 0.11–0.30 mg kg ?1 h at 25°C over 12 days thermal monitoring. Furthermore, amplicons cadA smt genes allow elucidation possible mechanisms used populations. gene amplification confirmed existence both bioweathering biochelating capacities. Scanning electron microscope (SEM) images supported otavite biologically induced naturally. A Pearson correlation analysis made kinetical growth parameters thermodynamic data. Besides, PCA performed copies, pH SOM indicating effect translocation. Thus, it concluded that combination obtained microcalorimetry, molecular basis immobilization, SEM imagery could act useful toolkit identify strategies contaminated farms. research importance study use combined tools quantitative measurements assess capacities soil, situ , new proxy assessment cacao-growing soils.